sn 001 Search Results


ifn genes inhibitor sn 001  (MedChemExpress)
94
MedChemExpress ifn genes inhibitor sn 001
Cisplatin increases the activity of the cGAS/STING pathway in a dose‐dependent manner. Knock‐in or knock‐down of the STING gene significantly increased or decreased PD‐L1 expression at protein and mRNA levels in GC cells. (A) Western blot analysis was conducted to assess the total and phosphorylation levels of TBK1, STING, and total level of cGAS in BGC‐823 (left) and MKN‐45 (right) cells following a 24‐h incubation with saline, 0.5 μg/ml cisplatin, or 2 μg/ml cisplatin. GAPDH was used as an internal control. (B and C) BGC‐823 cells were transfected with control or STING‐expressing plasmids. Expression levels of STING and PD‐L1 were determined by RT‐qPCR (B) and western blotting (C). ACTB and GAPDH served as internal controls, respectively. The relative mRNA levels were normalized to those of GFP‐expressing cells. (D and E) Cells transfected with STING‐specific siRNA (siSTING#1) or scrambled siRNA (siNC) were treated with 1 μg/ml cisplatin for another 36 h. RT‐qPCR (D) and western blotting (E) were used to measure the levels of STING and PD‐L1 expression. ACTB and GAPDH acted as internal controls, respectively. The relative mRNA levels of STING or PD‐L1 were normalized to those of siNC transfected cells (siNC). (F and G) BGC‐823 cells were pretreated with 10 <t>μM</t> <t>SN‐001</t> for 2 h followed by a 36‐h incubation with 1 μg/ml cisplatin. PD‐L1 expression was evaluated by RT‐qPCR (F) and western blotting (G), using ACTB and GAPDH as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. (H and I) BGC‐823 cells were pretreated with 10 m m NAC for 2 h, then incubated with 1 μg/ml cisplatin for another 36 h. Then, PD‐L1 expression was analyzed by RT‐qPCR (H) and western blotting (I), with ACTB and GAPDH serving as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. All statistical analyses were performed using a two‐tailed Student's t ‐test, and significance is indicated by * p < 0.05, ** p < 0.01, *** p < 0.001, and ns (not significant).
Ifn Genes Inhibitor Sn 001, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sn-001  (MuseChem Chemicals)
N/A
DUN99845(CAT: I026600) is a novel and selective inhibitor utilized in advanced biochemical and pharmaceutical research. It targets specific enzymatic pathways, making it valuable for studying signal transduction, cell cycle regulation, and metabolic processes. Known for
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bone tissue  (Invent Biotechnologies)
96
Invent Biotechnologies bone tissue
Cisplatin increases the activity of the cGAS/STING pathway in a dose‐dependent manner. Knock‐in or knock‐down of the STING gene significantly increased or decreased PD‐L1 expression at protein and mRNA levels in GC cells. (A) Western blot analysis was conducted to assess the total and phosphorylation levels of TBK1, STING, and total level of cGAS in BGC‐823 (left) and MKN‐45 (right) cells following a 24‐h incubation with saline, 0.5 μg/ml cisplatin, or 2 μg/ml cisplatin. GAPDH was used as an internal control. (B and C) BGC‐823 cells were transfected with control or STING‐expressing plasmids. Expression levels of STING and PD‐L1 were determined by RT‐qPCR (B) and western blotting (C). ACTB and GAPDH served as internal controls, respectively. The relative mRNA levels were normalized to those of GFP‐expressing cells. (D and E) Cells transfected with STING‐specific siRNA (siSTING#1) or scrambled siRNA (siNC) were treated with 1 μg/ml cisplatin for another 36 h. RT‐qPCR (D) and western blotting (E) were used to measure the levels of STING and PD‐L1 expression. ACTB and GAPDH acted as internal controls, respectively. The relative mRNA levels of STING or PD‐L1 were normalized to those of siNC transfected cells (siNC). (F and G) BGC‐823 cells were pretreated with 10 <t>μM</t> <t>SN‐001</t> for 2 h followed by a 36‐h incubation with 1 μg/ml cisplatin. PD‐L1 expression was evaluated by RT‐qPCR (F) and western blotting (G), using ACTB and GAPDH as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. (H and I) BGC‐823 cells were pretreated with 10 m m NAC for 2 h, then incubated with 1 μg/ml cisplatin for another 36 h. Then, PD‐L1 expression was analyzed by RT‐qPCR (H) and western blotting (I), with ACTB and GAPDH serving as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. All statistical analyses were performed using a two‐tailed Student's t ‐test, and significance is indicated by * p < 0.05, ** p < 0.01, *** p < 0.001, and ns (not significant).
Bone Tissue, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sn+001/Minute+Total+Protein+Extraction+Kit/pmc13007860-76-19-21
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sn(100) and (001) single crystal discs  (Leico Industries Inc)
90
Leico Industries Inc sn(100) and (001) single crystal discs
Cisplatin increases the activity of the cGAS/STING pathway in a dose‐dependent manner. Knock‐in or knock‐down of the STING gene significantly increased or decreased PD‐L1 expression at protein and mRNA levels in GC cells. (A) Western blot analysis was conducted to assess the total and phosphorylation levels of TBK1, STING, and total level of cGAS in BGC‐823 (left) and MKN‐45 (right) cells following a 24‐h incubation with saline, 0.5 μg/ml cisplatin, or 2 μg/ml cisplatin. GAPDH was used as an internal control. (B and C) BGC‐823 cells were transfected with control or STING‐expressing plasmids. Expression levels of STING and PD‐L1 were determined by RT‐qPCR (B) and western blotting (C). ACTB and GAPDH served as internal controls, respectively. The relative mRNA levels were normalized to those of GFP‐expressing cells. (D and E) Cells transfected with STING‐specific siRNA (siSTING#1) or scrambled siRNA (siNC) were treated with 1 μg/ml cisplatin for another 36 h. RT‐qPCR (D) and western blotting (E) were used to measure the levels of STING and PD‐L1 expression. ACTB and GAPDH acted as internal controls, respectively. The relative mRNA levels of STING or PD‐L1 were normalized to those of siNC transfected cells (siNC). (F and G) BGC‐823 cells were pretreated with 10 <t>μM</t> <t>SN‐001</t> for 2 h followed by a 36‐h incubation with 1 μg/ml cisplatin. PD‐L1 expression was evaluated by RT‐qPCR (F) and western blotting (G), using ACTB and GAPDH as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. (H and I) BGC‐823 cells were pretreated with 10 m m NAC for 2 h, then incubated with 1 μg/ml cisplatin for another 36 h. Then, PD‐L1 expression was analyzed by RT‐qPCR (H) and western blotting (I), with ACTB and GAPDH serving as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. All statistical analyses were performed using a two‐tailed Student's t ‐test, and significance is indicated by * p < 0.05, ** p < 0.01, *** p < 0.001, and ns (not significant).
Sn(100) And (001) Single Crystal Discs, supplied by Leico Industries Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sn+001/sn+100++and++001++single+crystal+discs/10__1016_slash_j__elecom__2011__08__026-21-1-10
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material (100) (001) î²-sn  (Verlag GmbH)
90
Verlag GmbH material (100) (001) î²-sn
Cisplatin increases the activity of the cGAS/STING pathway in a dose‐dependent manner. Knock‐in or knock‐down of the STING gene significantly increased or decreased PD‐L1 expression at protein and mRNA levels in GC cells. (A) Western blot analysis was conducted to assess the total and phosphorylation levels of TBK1, STING, and total level of cGAS in BGC‐823 (left) and MKN‐45 (right) cells following a 24‐h incubation with saline, 0.5 μg/ml cisplatin, or 2 μg/ml cisplatin. GAPDH was used as an internal control. (B and C) BGC‐823 cells were transfected with control or STING‐expressing plasmids. Expression levels of STING and PD‐L1 were determined by RT‐qPCR (B) and western blotting (C). ACTB and GAPDH served as internal controls, respectively. The relative mRNA levels were normalized to those of GFP‐expressing cells. (D and E) Cells transfected with STING‐specific siRNA (siSTING#1) or scrambled siRNA (siNC) were treated with 1 μg/ml cisplatin for another 36 h. RT‐qPCR (D) and western blotting (E) were used to measure the levels of STING and PD‐L1 expression. ACTB and GAPDH acted as internal controls, respectively. The relative mRNA levels of STING or PD‐L1 were normalized to those of siNC transfected cells (siNC). (F and G) BGC‐823 cells were pretreated with 10 <t>μM</t> <t>SN‐001</t> for 2 h followed by a 36‐h incubation with 1 μg/ml cisplatin. PD‐L1 expression was evaluated by RT‐qPCR (F) and western blotting (G), using ACTB and GAPDH as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. (H and I) BGC‐823 cells were pretreated with 10 m m NAC for 2 h, then incubated with 1 μg/ml cisplatin for another 36 h. Then, PD‐L1 expression was analyzed by RT‐qPCR (H) and western blotting (I), with ACTB and GAPDH serving as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. All statistical analyses were performed using a two‐tailed Student's t ‐test, and significance is indicated by * p < 0.05, ** p < 0.01, *** p < 0.001, and ns (not significant).
Material (100) (001) î² Sn, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sn-001  (Aladdin Scientific Corporation)
N/A
SN-001 is a STING inhibitor with an IC50 of 3.82 μM.
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sn-001  (TargetMol)
N/A
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Image Search Results


Cisplatin increases the activity of the cGAS/STING pathway in a dose‐dependent manner. Knock‐in or knock‐down of the STING gene significantly increased or decreased PD‐L1 expression at protein and mRNA levels in GC cells. (A) Western blot analysis was conducted to assess the total and phosphorylation levels of TBK1, STING, and total level of cGAS in BGC‐823 (left) and MKN‐45 (right) cells following a 24‐h incubation with saline, 0.5 μg/ml cisplatin, or 2 μg/ml cisplatin. GAPDH was used as an internal control. (B and C) BGC‐823 cells were transfected with control or STING‐expressing plasmids. Expression levels of STING and PD‐L1 were determined by RT‐qPCR (B) and western blotting (C). ACTB and GAPDH served as internal controls, respectively. The relative mRNA levels were normalized to those of GFP‐expressing cells. (D and E) Cells transfected with STING‐specific siRNA (siSTING#1) or scrambled siRNA (siNC) were treated with 1 μg/ml cisplatin for another 36 h. RT‐qPCR (D) and western blotting (E) were used to measure the levels of STING and PD‐L1 expression. ACTB and GAPDH acted as internal controls, respectively. The relative mRNA levels of STING or PD‐L1 were normalized to those of siNC transfected cells (siNC). (F and G) BGC‐823 cells were pretreated with 10 μM SN‐001 for 2 h followed by a 36‐h incubation with 1 μg/ml cisplatin. PD‐L1 expression was evaluated by RT‐qPCR (F) and western blotting (G), using ACTB and GAPDH as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. (H and I) BGC‐823 cells were pretreated with 10 m m NAC for 2 h, then incubated with 1 μg/ml cisplatin for another 36 h. Then, PD‐L1 expression was analyzed by RT‐qPCR (H) and western blotting (I), with ACTB and GAPDH serving as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. All statistical analyses were performed using a two‐tailed Student's t ‐test, and significance is indicated by * p < 0.05, ** p < 0.01, *** p < 0.001, and ns (not significant).

Journal: The Journal of Pathology: Clinical Research

Article Title: Platinum drugs upregulate CXCR4 and PD ‐ L1 expression via ROS ‐dependent pathways, with implications for novel combined treatment in gastric cancer

doi: 10.1002/2056-4538.70015

Figure Lengend Snippet: Cisplatin increases the activity of the cGAS/STING pathway in a dose‐dependent manner. Knock‐in or knock‐down of the STING gene significantly increased or decreased PD‐L1 expression at protein and mRNA levels in GC cells. (A) Western blot analysis was conducted to assess the total and phosphorylation levels of TBK1, STING, and total level of cGAS in BGC‐823 (left) and MKN‐45 (right) cells following a 24‐h incubation with saline, 0.5 μg/ml cisplatin, or 2 μg/ml cisplatin. GAPDH was used as an internal control. (B and C) BGC‐823 cells were transfected with control or STING‐expressing plasmids. Expression levels of STING and PD‐L1 were determined by RT‐qPCR (B) and western blotting (C). ACTB and GAPDH served as internal controls, respectively. The relative mRNA levels were normalized to those of GFP‐expressing cells. (D and E) Cells transfected with STING‐specific siRNA (siSTING#1) or scrambled siRNA (siNC) were treated with 1 μg/ml cisplatin for another 36 h. RT‐qPCR (D) and western blotting (E) were used to measure the levels of STING and PD‐L1 expression. ACTB and GAPDH acted as internal controls, respectively. The relative mRNA levels of STING or PD‐L1 were normalized to those of siNC transfected cells (siNC). (F and G) BGC‐823 cells were pretreated with 10 μM SN‐001 for 2 h followed by a 36‐h incubation with 1 μg/ml cisplatin. PD‐L1 expression was evaluated by RT‐qPCR (F) and western blotting (G), using ACTB and GAPDH as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. (H and I) BGC‐823 cells were pretreated with 10 m m NAC for 2 h, then incubated with 1 μg/ml cisplatin for another 36 h. Then, PD‐L1 expression was analyzed by RT‐qPCR (H) and western blotting (I), with ACTB and GAPDH serving as internal controls, respectively. The relative mRNA levels of PD‐L1 were normalized to those of vehicle‐incubated cells. All statistical analyses were performed using a two‐tailed Student's t ‐test, and significance is indicated by * p < 0.05, ** p < 0.01, *** p < 0.001, and ns (not significant).

Article Snippet: Intracellular reactive oxygen species (ROS) scavenger N‐acetylcysteine (NAC), and a stimulator of IFN genes inhibitor SN‐001 were purchased from MedChemExpress.

Techniques: Activity Assay, Knock-In, Knockdown, Expressing, Western Blot, Phospho-proteomics, Incubation, Saline, Control, Transfection, Quantitative RT-PCR, Two Tailed Test